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      首頁 > 產品中心 > 一抗 > 產品信息
      Rabbit Anti-Doublecortin antibody (bs-1269R)
      訂購QQ:  400-901-9800
      說明書: 50ul  100ul  200ul

      產品編號 bs-1269R
      英文名稱 Doublecortin
      中文名稱 雙皮質素抗體
      別    名 Doublecortex; DBCN; Dbct; DC; DCX; Doublin; Lis X; Lissencephalin X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; SCLH; XLIS.  
      Specific References  (3)     |     bs-1269R has been referenced in 3 publications.
      [IF=1.98] Xu HY et al. Protective Effect of Mesenchymal Stromal Cell-Derived Exosomes on Traumatic Brain Injury via miR-216a-5p. Med Sci Monit. 2020; 26: e920855-1–e920855-12.  IF ;  rat.  
      [IF=1.329] Gao N et al. Volatile Oil from Acorus gramineus Ameliorates the Injury Neurons in the Hippocampus of Amyloid Beta 1–42 Injected Mice. Anat Rec (Hoboken). 2019 Aug 23.  WB&IHC-P ;  Mouse.  
      [IF=3.568] Salois and Smith Housing Complexity Alters GFAP-Immunoreactive Astrocyte Morphology in the Rat Dentate Gyrus. (2016) Neural.Plas. 2016:3928726  IF(IHC-P) ;  Rat.  
      研究領域 細胞生物  神經生物學  細胞粘附分子  細胞類型標志物  
      抗體來源 Rabbit
      克隆類型 Polyclonal
      交叉反應 Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, )
      產品應用 WB=1:500-2000 ELISA=1:5000-10000 ICC=1:100 
      not yet tested in other applications.
      optimal dilutions/concentrations should be determined by the end user.
      理論分子量 49kDa
      細胞定位 細胞漿 
      性    狀 Liquid
      濃    度 1mg/ml
      免 疫 原 KLH conjugated synthetic peptide derived from human Doublecortin: 31-150/362 
      亞    型 IgG
      純化方法 affinity purified by Protein A
      緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
      保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
      注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
      PubMed PubMed
      產品介紹 Neuronal Marker

      Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis.

      Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration.

      Interacts with tubulin.

      Subcellular Location:
      Cytoplasm. Cell projection. Note=Localizes at neurite tips.

      Tissue Specificity:
      Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

      Post-translational modifications:
      Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind mirotubules.

      Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
      Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
      Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).

      Contains 2 doublecortin domains.


      Gene ID:

      Database links:

      Entrez Gene: 1641 Human

      Entrez Gene: 13193 Mouse

      Entrez Gene: 84394 Rat

      Omim: 300121 Human

      SwissProt: O43602 Human

      SwissProt: O88809 Mouse

      SwissProt: Q9ESI7 Rat

      Unigene: 34780 Human

      Unigene: 12871 Mouse

      Unigene: 121471 Rat

      神經細胞標志物(Neuronal Marker)
      Embryo (mouse) Lysate at 40 ug
      Cerebrum (mouse) Lysate at 40 ug
      Cerebrum (Rat) Lysate at 40 ug
      Heart (mouse) Lysate at 40 ug
      Primary: Anti-Doublecortin (bs-1269R)at 1/300 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 49kD
      Observed band size: 49 kD
      Lane 1: Cerebrum (Mouse) Lysate at 40 ug
      Lane 2: Cerebrum (Rat) Lysate at 40 ug
      Primary: Anti-Doublecortin (bs-1269R) at 1/1000 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 45 kD
      Observed band size: 47 kD
      SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Doublecortin) polyclonal Antibody, Unconjugated (bs-1269R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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