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      RabbitAnti-ChAT antibody (bs-0042R)
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      產品編號 bs-0042R
      英文名稱 ChAT
      中文名稱 ChAT膽堿乙酰轉移酶抗體
      別    名 Choline O acetyltransferase; Choline O acetyltransferase; Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584; CLAT_HUMAN.  
      Specific References  (11)     |     bs-0042R has been referenced in 11 publications.
      [IF=5.135] Wei Lu. et al. Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transection. Neural Regen Res. 2022 Jan;17(8):1827  IHC ;  Rat.  
      [IF=3.856] Daisuke Koga. et al. Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure. Front Neuroanat. 2021; 15: 759804  IF ;  Rat.  
      [IF=2.74] Jiasuoer Xiaokereti. et al. Enhanced atrial internal-external neural remodeling facilitates atrial fibrillation in the chronic obstructive sleep apnea model. Plos One. 2021 Feb;16(2):e0247308  WB,IHC ;  Dog.  
      [IF=3.842] Chen L et al. Enteric motor dysfunctions in experimental chronic pancreatitis: Alterations of myenteric neurons regulating colonic motility in rats. Neurogastroenterol Motil. 2018 Jul;30(7):e13301.  IF ;  Rat.  
      [IF=3.536] Zhang,et al.Effects of C1 inhibitor on endothelial cell activation in a rat hind limb ischemia-reperfusion injury model.(2018) Journal of Vascular Surgery. :.  IF(IHC-F) ;  Rat.  
      [IF=3.508] Wieck et al. Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic. (2016) Tissue.Eng.Part.A. 22:53-64  IHC-P ;  Human.  
      [IF=3.58] Zhang, Xiuli, et al. "Catalpol improves cholinergic function and reduces inflammatory cytokines in the senescent mice induced by D-galactose."Food and chemical toxicology 58 (2013): 50-55.  IHC-P ;  Mouse.  
      [IF=4.45] Wieck, Minna M., et al. "Human and murine tissue-engineered colon exhibit diverse neuronal subtypes and can be populated by enteric nervous system progenitor cells when donor colon is aganglionic." Tissue Engineering A (2015).  IHC-P ;  Human.  
      [IF=2.014] Li LI. et al. The antibacterial activity of Berberis heteropoda Schrenk and its effect on irritable bowel syndrome in rats. Chin J Nat Medicines. 2020 May;18:356  IHC ;  Rat.  
      [IF=4.259] Wang,et al.Stimulation of Epicardial Sympathetic Nerves at Different Sites Induces Cardiac Electrical Instability to Various Degrees.(2018) Scientific Reports. 8:994.  IHC-P ;  Dog.  
      [IF=3.53] Zhang Y, Zheng S, Geng Y, Xue J, Wang Z, et al. (2015) MicroRNA Profiling of Atrial Fibrillation in Canines: MiR-206 Modulates Intrinsic Cardiac Autonomic Nerve Remodeling by Regulating SOD1. PLoS ONE 10(3): e0122674.  other ;  Dog.  
      研究領域 神經生物學  Alzheimer's  
      抗體來源 Rabbit
      克隆類型 Polyclonal
      交叉反應 Human, Mouse, Rat,  (predicted: Dog, Pig, )
      產品應用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:200-800 (石蠟切片需做抗原修復)
      not yet tested in other applications.
      optimal dilutions/concentrations should be determined by the end user.
      理論分子量 82kDa
      細胞定位 細胞核 細胞漿 
      性    狀 Liquid
      濃    度 1mg/ml
      免 疫 原 KLH conjugated synthetic peptide derived from human ChAT: 101-200/748 
      亞    型 IgG
      純化方法 affinity purified by Protein A
      緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
      保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
      注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
      PubMed PubMed
      產品介紹 This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010].

      Function:
      Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

      DISEASE:
      Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.

      Similarity:
      Belongs to the carnitine/choline acetyltransferase family.

      SWISS:
      P28329

      Gene ID:
      1103

      Database links:

      Entrez Gene: 1103 Human

      Entrez Gene: 12647 Mouse

      Entrez Gene: 290567 Rat

      Omim: 118490 Human

      SwissProt: P28329 Human

      SwissProt: Q03059 Mouse

      SwissProt: P32738 Rat

      Unigene: 302002 Human

      Unigene: 442817 Mouse

      Unigene: 45116 Rat



      膽堿乙酰轉移酶是一種在神經元胞體內合成的酶。當該轉移酶被合成以后,通過軸質流動方式轉移到神經軸突末端。其功能是將乙酰輔酶A轉移到膽堿上,導致神經遞質乙酰膽堿的形成。膽堿能系統參與多種神經功能。一些膽堿能神經元的改變能導致阿爾茨海默病的發生。
      膽堿乙酰轉移酶通常被用來標記神經元。
      產品圖片
      Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated (bs-0042R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
      Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, unconjugated (bs-0042R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
      Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
      Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
      Incubation: Anti-ChAT Polyclonal Antibody, Unconjugated(bs-0042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
      Blank control:Molt-4.
      Primary Antibody (green line): Rabbit Anti-ChAT antibody (bs-0042R)
      Dilution: 1μg /10^6 cells;
      Isotype Control Antibody (orange line): Rabbit IgG .
      Secondary Antibody : Goat anti-rabbit IgG-AF647
      Dilution: 1μg /test.
      Protocol
      The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
      Blank control (Black line): Molt-4(Black).
      Primary Antibody (green line): Rabbit Anti-ChAT antibody (bs-0042R)
      Dilution: 1μg /10^6 cells;
      Isotype Control Antibody (orange line): Rabbit IgG .
      Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
      Dilution: 1μg /test.
      Protocol
      The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
      Blank control: SH-SY5Y.
      Primary Antibody (green line): Rabbit Anti-ChAT antibody (bs-0042R)
      Dilution: 1μg /10^6 cells;
      Isotype Control Antibody (orange line): Rabbit IgG .
      Secondary Antibody : Goat anti-rabbit IgG-AF647
      Dilution: 1μg /test.
      Protocol
      The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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