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      bs-9360R [Primary Antibody]
      Rabbit  Anti-Proteasome 20S alpha 1 Polyclonal Antibody
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      DATASHEET

      Host: Rabbit

      Target Protein: Proteasome 20S alpha 1

      IR: Immunogen Range:1-100/263

      Clonality: Polyclonal

      Isotype: IgG

      Entrez Gene: 5682

      Swiss Prot: P25786

      Source: KLH conjugated synthetic peptide derived from human Proteasome 20S alpha:1-100/263 

      Purification: affinity purified by Protein A

      Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

      Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

      Size: 50ul

      Concentration: 1mg/ml

      Applications: ELISA(1:5000-10000)
      IHC-P(1:100-500)
      IHC-F(1:100-500)
      IF(1:50-200)

      Cross Reactive Species: Human
      Mouse
      Rat
      .

      For research use only. Not intended for diagnostic or therapeutic use.

      VALIDATION IMAGES
      Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Proteasome 20S alpha + beta) Polyclonal Antibody, Unconjugated (bs-9360R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
      Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Proteasome 20S alpha + beta) Polyclonal Antibody, Unconjugated (bs-9360R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
      Paraformaldehyde-fixed, paraffin embedded (Human smooth muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Proteasome 20S alpha + beta) Polyclonal Antibody, Unconjugated (bs-9360R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
       
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